Q fever

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Q fever, a zoonotic disease caused by the Gram-negative bacteria Coxiella burnetti, is spread by parturient cattle.

C. burnetii is a parasite of bovine phagocytes, closely related to Francisella spp.

It resides and reproduces in phagolysosomes of host monocytes and macrophages. Two forms exist—the large cell variant is a vegetative form found in infected cells, and the small cell variant is the extracellular infectious form that is shed in milk, urine, and feces and found in high concentration (109 ID50/g) in placental tissue and amniotic fluid. The small cell variant is resistant to heat, drying, and many common disinfectants and remains viable for weeks to months in the environment. Once a domestic ruminant is infected, C burnetii can localize in mammary glands, supramammary lymph nodes, placenta, and uterus, from which it may be shed in subsequent parturitions and lactations.

The epidemiology of C burnetii is complex because there are 2 major patterns of transmission: in one, the organism circulates between wild animals and their ectoparasites, mainly ticks; the other occurs in domestic ruminants, independent of the wild animal cycle. Ixodid and argasid ticks can act as reservoirs of the organism. Distribution is worldwide (except New Zealand), and the host range includes various wild and domestic mammals, arthropods, and birds. The disease is enzootic in most areas where cattle, sheep, and goats are kept. In the USA, seroprevalence studies have shown antibodies to C burnetii in 41.6% of sheep, 16.5% of goats, and 3.4% of cattle.

The greatest risk of transmission occurs at parturition by inhalation, ingestion, or direct contact with birth fluids or placenta. The organism is also shed in milk, urine, and feces. High-temperature pasteurization effectively kills the organism. Ticks may transmit the disease among domestic ruminants but are not thought to play an epidemiologically important role in transmission of disease to humans.

Clinical Findings and Diagnosis

Infection in ruminants is usually subclinical but can cause anorexia and late abortion. Reports have implicated C burnetii as a cause of infertility and sporadic abortion with a necrotizing placentitis in ruminants. New evidence has shown an association of C burnetii with subclinical mastitis among dairy cows. Experimental infection in cats causes transient fever, dullness, and anorexia lasting several days.

In domestic ruminants, gross lesions are nonspecific, and differential diagnosis should include infectious and noninfectious agents that cause abortion. Immunofluorescence test on paired sera taken ≥2 wk apart can be used to detect recent infection; however, shedding of C burnetii may occur in the absence of a measurable serum antibody titer. Culture, immunohistochemical, and PCR tests may be used to identify the organism in tissues.

Treatment and Control

Q fever in humans is a notifiable disease in the USA, primarily because of its status as a possible bioterrorism agent; reporting is not usually required for animals unless associated with human infection. Vaccines for people and animals have been developed but are not commercially available in the USA. Vaccination has prevented infection when administered to uninfected calves and has improved fertility and reduced shedding in previously infected animals.

For treatment of ruminants, oral tetracycline at the therapeutic dose may be given for 2–4 wk. In known infected herds, segregating pregnant animals indoors, burning or burying reproductive offal, or administering tetracycline (8 mg/kg/day) prophylactically in the water supply prior to parturition may reduce spread of the organism.

Zoonotic Risk

Q fever occurs more frequently in persons who have occupational contact with high-risk species. It has a highly variable clinical presentation in humans, ranging from a self-limiting influenza-like illness to pneumonia, hepatitis, and endocarditis. It is highly infectious, and a single organism can reportedly cause infection via the aerosol route in humans.

The majority of outbreaks in people have been associated with wind dispersion of desiccated reproductive products, contaminated with C burnetii, from sites where sheep, goats, or cattle are kept. Farmers and veterinarians are at risk while assisting birthing. Slaughterhouse workers are at risk from contact with infected carcasses, hair, and wool. Transmission may also occur by consumption of unpasteurized milk. Handling of infected tissue poses a threat to laboratory personnel. Q fever has been seen in personnel and human patients in medical institutions where latently infected sheep were used for research. Medical facilities using ruminants in research should purchase animals from Q fever-free flocks and use males when possible. In addition, workers should use adequate personal protective equipment to protect against small droplet and aerosol exposure during high-risk medical procedures.